O-148 Cumulative embryotoxic effect of IVF plastic devices used sequentially in routine clinical practice could slow the embryo development and delay blastulation

Abstract Study question Is there an embryotoxic cumulative effect resulting from IVF plastic consumables (PC) when used sequentially under clinical routine practice? Summary answer Reduced blastulation rates and slow development were detected in some associations of PC evaluated by the Mouse Embryo Assay (MEA) suggesting possible embryotoxicity. What is known already Recommendations European (ESHRE) American (ASRM) societies regarding use suggest that they should undergo appropriate quality control tests to detect presence embryotoxins. The main test manufacturers exclude any embryotoxicity MEA. This assay currently performed individually on each so a certificate conformity delivered for product/lot. However, several are during single cycle, potentially creating effect. To our knowledge, toxicity gametes embryos has not been studied far. design, size, duration objective this prospective study was determine if together conditions. Ten associations, containing 13 31 PC, designed, assessed using MEA methodology. tested combinations replicated different steps including sperm collection selection, oocyte pick-up, fertilization, embryo culture, transfer, vitrification thawing freezing. Participants/materials, setting, methods A defined volume culture media extract every association triplicate. medium extraction with 21 one-cell stage fresh mouse embryos. Blastocyst formation after 96 120h (Day 5/6), blastocyst good hatched compared between groups. total cell number per also evaluated. Main results role chance No first two comprised 22 23 which mimicked (either cups or spermicide-free condoms) processing samples. third 32 devices replicating cups, processing, freezing high security straws, showed as mean rate at day 5 reduced 20.6% 95.6% group (p < 0.05). Surprisingly, observed 6 55.6% versus 100.0% blastocysts obtained cells (n = 112.5) significantly comparison 167.9) 0.05), sublethal toxins slowed down delayed blastulation. More experiments involving assessment other undergoing will be presented conference. Limitations, reasons caution Sensitivity could vary depending methodologies cause, any, need pinpointed analyzing element affected individually. Wider implications findings Also, professionals rationalize minimize procedures reduce potential accumulation system. Trial registration Not applicable